Gene description for CD63
Gene name CD63 molecule
Gene symbol CD63
Other names/aliases AD1
HOP-26
ME491
MLA1
OMA81H
Pltgp40
TSPAN30
Species Homo sapiens
 Experiment description of studies that identified CD63 in Monocytic leukemia cells

1
Experiment ID2486
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 34388111   
OrganismHomo sapiens
Experiment descriptionmiRNA-221-3p derived from M2-polarized tumor-associated macrophage exosomes aggravates the growth and metastasis of osteosarcoma through SOCS3/JAK2/STAT3 axis
AuthorsLiu W, Long Q, Zhang W, Zeng D, Hu B, Liu S, Chen L.
Journal name Aging
Publication year2021
SampleMonocytic leukemia cells
Sample nameTHP1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyWestern blotting
qRT-PCR
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2
Experiment ID2487
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 34388111   
OrganismHomo sapiens
Experiment descriptionmiRNA-221-3p derived from M2-polarized tumor-associated macrophage exosomes aggravates the growth and metastasis of osteosarcoma through SOCS3/JAK2/STAT3 axis
AuthorsLiu W, Long Q, Zhang W, Zeng D, Hu B, Liu S, Chen L.
Journal name Aging
Publication year2021
SampleMonocytic leukemia cells
Sample nameTHP1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyWestern blotting
qRT-PCR
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3
Experiment ID2488
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 34388111   
OrganismHomo sapiens
Experiment descriptionmiRNA-221-3p derived from M2-polarized tumor-associated macrophage exosomes aggravates the growth and metastasis of osteosarcoma through SOCS3/JAK2/STAT3 axis
AuthorsLiu W, Long Q, Zhang W, Zeng D, Hu B, Liu S, Chen L.
Journal name Aging
Publication year2021
SampleMonocytic leukemia cells
Sample nameTHP1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyWestern blotting
qRT-PCR
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4
Experiment ID2489
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 34388111   
OrganismHomo sapiens
Experiment descriptionmiRNA-221-3p derived from M2-polarized tumor-associated macrophage exosomes aggravates the growth and metastasis of osteosarcoma through SOCS3/JAK2/STAT3 axis
AuthorsLiu W, Long Q, Zhang W, Zeng D, Hu B, Liu S, Chen L.
Journal name Aging
Publication year2021
SampleMonocytic leukemia cells
Sample nameTHP1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyWestern blotting
qRT-PCR
EV-TRACK -

5
Experiment ID2490
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 34388111   
OrganismHomo sapiens
Experiment descriptionmiRNA-221-3p derived from M2-polarized tumor-associated macrophage exosomes aggravates the growth and metastasis of osteosarcoma through SOCS3/JAK2/STAT3 axis
AuthorsLiu W, Long Q, Zhang W, Zeng D, Hu B, Liu S, Chen L.
Journal name Aging
Publication year2021
SampleMonocytic leukemia cells
Sample nameTHP1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyWestern blotting
qRT-PCR
EV-TRACK -

6
Experiment ID1670
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Flow cytometry
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SampleMonocytic leukemia cells
Sample nameTHP-1
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
mRNA
Methods used in the studyRT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
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7
Experiment ID2502
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Flow cytometry
PubMed ID 34322205   
OrganismHomo sapiens
Experiment descriptionMolecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin
AuthorsVeerman RE, Teeuwen L, Czarnewski P, Güclüler Akpinar G, Sandberg A, Cao X, Pernemalm M, Orre LM, Gabrielsson S, Eldh M.
Journal name J Extracell Vesicles
Publication year2021
SampleMonocytic leukemia cells
Sample nameMM6 - ExoQuick Ultra
Isolation/purification methodsExoQuick Kit
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyFlow cytometry
Mass spectometry
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8
Experiment ID2503
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Flow cytometry
PubMed ID 34322205   
OrganismHomo sapiens
Experiment descriptionMolecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin
AuthorsVeerman RE, Teeuwen L, Czarnewski P, Güclüler Akpinar G, Sandberg A, Cao X, Pernemalm M, Orre LM, Gabrielsson S, Eldh M.
Journal name J Extracell Vesicles
Publication year2021
SampleMonocytic leukemia cells
Sample nameMM6 - exoEasy
Isolation/purification methodsexoEasy Maxi Kit
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyFlow cytometry
Mass spectometry
EV-TRACK -

9
Experiment ID2504
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Flow cytometry
PubMed ID 34322205   
OrganismHomo sapiens
Experiment descriptionMolecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin
AuthorsVeerman RE, Teeuwen L, Czarnewski P, Güclüler Akpinar G, Sandberg A, Cao X, Pernemalm M, Orre LM, Gabrielsson S, Eldh M.
Journal name J Extracell Vesicles
Publication year2021
SampleMonocytic leukemia cells
Sample nameMM6 - iZON 35 nm column
Isolation/purification methodsSize exclusion chromatography
Centrifugal ultrafiltration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyFlow cytometry
Mass spectometry
EV-TRACK -

10
Experiment ID2505
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVLarge EVs
Identification method Flow cytometry
PubMed ID 34322205   
OrganismHomo sapiens
Experiment descriptionMolecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin
AuthorsVeerman RE, Teeuwen L, Czarnewski P, Güclüler Akpinar G, Sandberg A, Cao X, Pernemalm M, Orre LM, Gabrielsson S, Eldh M.
Journal name J Extracell Vesicles
Publication year2021
SampleMonocytic leukemia cells
Sample nameMM6 - iZON 70 nm column
Isolation/purification methodsSize exclusion chromatography
Centrifugal ultrafiltration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyFlow cytometry
Mass spectometry
EV-TRACK -

11
Experiment ID2506
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Flow cytometry
PubMed ID 34322205   
OrganismHomo sapiens
Experiment descriptionMolecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin
AuthorsVeerman RE, Teeuwen L, Czarnewski P, Güclüler Akpinar G, Sandberg A, Cao X, Pernemalm M, Orre LM, Gabrielsson S, Eldh M.
Journal name J Extracell Vesicles
Publication year2021
SampleMonocytic leukemia cells
Sample nameMM6 - OptiPrep
Isolation/purification methodsOptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density1.12–1.19 g/mL
Molecules identified in the studyProtein
Methods used in the studyFlow cytometry
Mass spectometry
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12
Experiment ID2507
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Flow cytometry
PubMed ID 34322206   
OrganismHomo sapiens
Experiment descriptionMolecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin
AuthorsVeerman RE, Teeuwen L, Czarnewski P, Güclüler Akpinar G, Sandberg A, Cao X, Pernemalm M, Orre LM, Gabrielsson S, Eldh M.
Journal name J Extracell Vesicles
Publication year2021
SampleMonocytic leukemia cells
Sample nameMM6 - MagCapture
Isolation/purification methodsMagCapture Exosome Isolation Kit PS
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyFlow cytometry
Mass spectometry
EV-TRACK -

13
Experiment ID2508
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Flow cytometry
PubMed ID 34322205   
OrganismHomo sapiens
Experiment descriptionMolecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin
AuthorsVeerman RE, Teeuwen L, Czarnewski P, Güclüler Akpinar G, Sandberg A, Cao X, Pernemalm M, Orre LM, Gabrielsson S, Eldh M.
Journal name J Extracell Vesicles
Publication year2021
SampleMonocytic leukemia cells
Sample nameMM6 - iZON 35 nm column flow-through fraction
Isolation/purification methodsSize exclusion chromatography
Centrifugal ultrafiltration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyFlow cytometry
Mass spectometry
EV-TRACK -

14
Experiment ID2509
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Flow cytometry
PubMed ID 34322205   
OrganismHomo sapiens
Experiment descriptionMolecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin
AuthorsVeerman RE, Teeuwen L, Czarnewski P, Güclüler Akpinar G, Sandberg A, Cao X, Pernemalm M, Orre LM, Gabrielsson S, Eldh M.
Journal name J Extracell Vesicles
Publication year2021
SampleMonocytic leukemia cells
Sample nameMM6 - iZON 70 nm column flow-through fraction
Isolation/purification methodsSize exclusion chromatography
Centrifugal ultrafiltration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyFlow cytometry
Mass spectometry
EV-TRACK -

15
Experiment ID2511
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Flow cytometry
PubMed ID 34322205   
OrganismHomo sapiens
Experiment descriptionMolecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin
AuthorsVeerman RE, Teeuwen L, Czarnewski P, Güclüler Akpinar G, Sandberg A, Cao X, Pernemalm M, Orre LM, Gabrielsson S, Eldh M.
Journal name J Extracell Vesicles
Publication year2021
SampleMonocytic leukemia cells
Sample nameMM6 - OptiPrep low density fraction
Isolation/purification methodsOptiPrep density gradient centrifugation
Ultracentrifugation
Flotation densityTwo closest fractions below 1.12 g/mL
Molecules identified in the studyProtein
Methods used in the studyFlow cytometry
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